Review



lysosome associated membrane protein 2  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech lysosome associated membrane protein 2
    Lysosome Associated Membrane Protein 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Antibody/pmc12220713-42-80-86
    Average 96 stars, based on 219 article reviews
    lysosome associated membrane protein 2 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Long-term leucine supplementation increases body weight in goats by controlling appetite and muscle protein synthesis under protein-restricted conditions
    Article Snippet: .. 294 Primary antibody: mouse mTOR (1:50, Proteintech, 66888-1-Ig); rabbit lysosomal 295 associated membrane protein 2 (LAMP2) (1:50, Proteintech, 10397-1-AP). .. Secondary 296 antibodies: donkey anti-mouse IgG (H + L) (1:200, Proteintech, SA00013-7); donkey 297 anti-rabbit IgG (H + L) (1:200, Proteintech, SA00013-6) were used.

    Article Title: Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
    Article Snippet: HRP-conjugated goat anti-mouse IgG (SE131) and the CCK-8 cell proliferation and cytotoxicity assay kit (CA1210) were acquired from Solarbio (Beijing, China). .. Primary antibodies against β-actin (66009-1-Ig), p38 MAPK (66234-4-Ig), lysosome-associated membrane protein 2 (LAMP2) (66301-1), and extracellular signal-regulated kinases 1 and 2 (Erk1/2) (16443-1-AP) and fluorescent secondary antibodies (CoraLite594-conjugated donkey anti-rabbit IgG [SA00006-8] and Alexa Fluor 488-conjugated AffiniPure goat anti-mouse IgG [SA00006-1]) were obtained from Proteintech (Wuhan, China). ..

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Article Title: Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages.
    Article Snippet: HRPconjugated goat anti-mouse IgG (SE131) and the CCK-8 cell proliferation and cytotoxicity assay kit (CA1210) were acquired from Solarbio (Beijing, China). .. Primary antibodies against β-actin (66009-1-Ig), p38 MAPK (66234-4-Ig), lysosome-associated membrane protein 2 (LAMP2) (66301-1), and extracellular signal-regulated kinases 1 and 2 (Erk1/2) (16443-1-AP) and fluorescent secondary antibodies (CoraLite594-conjugated donkey anti-rabbit IgG [SA00006-8] and Alexa Fluor 488-conjugated AffiniPure goat anti-mouse IgG [SA00006-1]) were obtained from Proteintech (Wuhan, China). ..

    Incubation:

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Fluorescence:

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Microscopy:

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.



    Similar Products

    8 1e7 s lysosome associated membrane protein 2 lamp2  (Developmental Studies Hybridoma Bank)
    91
    Developmental Studies Hybridoma Bank 8 1e7 s lysosome associated membrane protein 2 lamp2
    8 1e7 S Lysosome Associated Membrane Protein 2 Lamp2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/anti-Glial+fibrillary+acidic+protein/pmc11910108__mmc1-4-10-9
    Average 91 stars, based on 1 article reviews
    8 1e7 s lysosome associated membrane protein 2 lamp2 - by Bioz Stars, 2026-10
    91/100 stars
    		  Buy from Supplier
    lysosomal associated membrane protein 2 lamp2  (Huabio Inc)
    86
    Huabio Inc lysosomal associated membrane protein 2 lamp2
    Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with <t>LAMP2</t> after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.
    Lysosomal Associated Membrane Protein 2 Lamp2, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/anti+cyclin+d1/pmc13070684-58-17-28
    Average 86 stars, based on 1 article reviews
    lysosomal associated membrane protein 2 lamp2 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    lysosomal associated membrane protein 2 lamp2 antibody  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology lysosomal associated membrane protein 2 lamp2 antibody
    Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with <t>LAMP2</t> after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.
    Lysosomal Associated Membrane Protein 2 Lamp2 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2a+Rabbit+mAb/pm42019387-92-35-43
    Average 94 stars, based on 1 article reviews
    lysosomal associated membrane protein 2 lamp2 antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    rabbit anti lysosomal associated membrane protein 2 lamp2 monoclonal antiserum  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc rabbit anti lysosomal associated membrane protein 2 lamp2 monoclonal antiserum
    Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with <t>LAMP2</t> after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.
    Rabbit Anti Lysosomal Associated Membrane Protein 2 Lamp2 Monoclonal Antiserum, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Rabbit+mAb/pm41820376-270-56-64
    Average 95 stars, based on 1 article reviews
    rabbit anti lysosomal associated membrane protein 2 lamp2 monoclonal antiserum - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    lysosome associated membrane protein 2  (Proteintech)
    96
    Proteintech lysosome associated membrane protein 2
    Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with <t>LAMP2</t> after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.
    Lysosome Associated Membrane Protein 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Antibody/pmc12220713-42-80-86
    Average 96 stars, based on 1 article reviews
    lysosome associated membrane protein 2 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    anti lysosomal associated membrane protein 2 lamp2 antibody  (Proteintech)
    96
    Proteintech anti lysosomal associated membrane protein 2 lamp2 antibody
    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins <t>LAMP2</t> and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.
    Anti Lysosomal Associated Membrane Protein 2 Lamp2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Antibody/pmc12833329-40-3-40
    Average 96 stars, based on 1 article reviews
    anti lysosomal associated membrane protein 2 lamp2 antibody - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    membrane protein 2 lamp2  (Proteintech)
    96
    Proteintech membrane protein 2 lamp2
    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins <t>LAMP2</t> and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.
    Membrane Protein 2 Lamp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Antibody/pm40517353-84-12-17
    Average 96 stars, based on 1 article reviews
    membrane protein 2 lamp2 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    anti-lysosomal-associated membrane protein 2 lamp2  (Affinity Biosciences)
    90
    Affinity Biosciences anti-lysosomal-associated membrane protein 2 lamp2
    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins <t>LAMP2</t> and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.
    Anti Lysosomal Associated Membrane Protein 2 Lamp2, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/lamp2+antibody/pm40319239-105-33-38
    Average 90 stars, based on 1 article reviews
    anti-lysosomal-associated membrane protein 2 lamp2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    lysosomal associated membrane protein 2  (Cusabio)
    93
    Cusabio lysosomal associated membrane protein 2
    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins <t>LAMP2</t> and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.
    Lysosomal Associated Membrane Protein 2, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/Rabbit+anti-Human+LAMP2+Polyclonal+Antibody/pmc11837466-54-44-53
    Average 93 stars, based on 1 article reviews
    lysosomal associated membrane protein 2 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    lysosomal associated membrane protein 2  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology lysosomal associated membrane protein 2
    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins <t>LAMP2</t> and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.
    Lysosomal Associated Membrane Protein 2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+protein+2+lamp2/LAMP2+Rabbit+pAb/pm40120344-85-34-41
    Average 95 stars, based on 1 article reviews
    lysosomal associated membrane protein 2 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with LAMP2 after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.

    Journal: Precision Clinical Medicine

    Article Title: Agrimol B inhibits pancreatic ductal adenocarcinoma by induction of lethal mitophagy through decreasing mitochondrial transcription termination factor 3

    doi: 10.1093/pcmedi/pbag009

    Figure Lengend Snippet: Agrimol B blocks autophagic flux in PDAC cells. (A, B) Western blot analysis of P62 and CTSD in PANC-1 and AsPC-1 cells treated with Agrimol B for 24 h. (C, E, F) Immunofluorescence analysis of RFP-GFP-LC3 after PANC-1 and AsPC-1 cells were transfected with RFP-GFP-LC3 for 48 h, followed by treatment with or without Agrimol B for another 24 h. Scale bars, 10 μm. (D, G-L) Immunofluorescence analysis of the colocalization of endogenous LC3 with LAMP2 after treatment with Agrimol B or rapamycin for 24 h in PANC-1 and AsPC-1 cells. Scale bars, 10 μm. (M-O) Immunofluorescence analysis of LC3 in PDAC cells treated with or without Agrimol B in the presence or absence of HCQ. Scale bars, 10 μm.

    Article Snippet: An anti-microtubule-associated protein light chain 3 (LC3) antibody (NB100-2220) was purchased from Novus, while anti-MTERF3 (EM1701-29) and lysosomal associated membrane protein 2 (LAMP2) (M1603-5) antibodies were purchased from HuaBio.

    Techniques: Western Blot, Immunofluorescence, Transfection

    Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins LAMP2 and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fenofibrate suppresses Mycoplasma bovis infection via autophagy-mediated cholesterol regulation in bovine mammary epithelial cells and murine mammary tissue

    doi: 10.3389/fcimb.2025.1731492

    Figure Lengend Snippet: Effects of doses and treatment durations of fenofibrate on autophagy by bMECs. (A) bMECs were treated with 50 μM fenofibrate for 0, 3, 6, 9, or 12 h, with HBSS serving as a positive control. Expression levels of lysosome-associated proteins LAMP2 and RAB7A, along with autophagy markers LC3 and SQSTM1, were assessed by Western blotting. Densitometric analysis was performed to quantify protein expression, normalized to GAPDH as a loading control. (B) bMECs were treated with 0, 10, 50, 100, or 200 μM fenofibrate for 9 h, with HBSS as a positive control. Protein levels of LAMP2, RAB7A, LC3, and SQSTM1 were evaluated by Western blotting. Relative expression was quantified by densitometry and normalized to GAPDH (for (A, B) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used). Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: Anti-LAMP1 antibody (67300-1-Ig), anti- lysosomal-associated membrane protein 2 (LAMP2) antibody (66301-1-Ig), anti-TFEB antibody (13372-1-AP), anti-TFE3 antibody (14480-1-AP), anti-LC3 polyclonal antibody (14600-1-AP), anti-ATG5 antibody (10181-2-AP), anti-RAB7A antibody (55469-1-AP), anti-mouse IgG-horseradish peroxidase (HRP) (SA00001-1), and Goat anti-rabbit IgG (SA00001-2) were all from Proteintech (Chicago, IL, USA).

    Techniques: Positive Control, Expressing, Western Blot, Control

    Fenofibrate restores autophagic activity and autophagic flux in bMECs infected with M. bovis . (A) bMECs were divided into 6 experimental groups: control, M. bovis PG45 reference strain infection (MOI = 30, 9 hpi), M. bovis WT21 wild-type strain infection (MOI = 30, 9 hpi), fenofibrate treatment alone, fenofibrate combined with PG45 infection, and fenofibrate combined with WT21 infection. Expression levels of LAMP2, RAB7A, SQSTM1, and LC3, along with the loading control GAPDH, were evaluated by Western blot (using specific antibodies). (B) Densitometric analysis was performed to quantify relative protein levels of LAMP2, RAB7A, SQSTM1, and LC3-II, normalized to GAPDH. (C) Representative confocal images depict autophagic flux in mCherry-GFP-LC3-transfected bMECs across the 6 treatment groups: control, PG45-infected, WT21-infected, fenofibrate-treated, fenofibrate+PG45, and fenofibrate+WT21. Yellow puncta indicate autophagosomes, whereas red puncta represent autolysosomes. Nuclei were counterstained with Hoechst 33258 (blue). Scale bar = 10 μm. (D) Quantification of autophagosomes in bMECs. Twenty cells for each sample and at least 60 cells in each group were used for statistical analyses, Superscript ‘a’: Yellow puncta compared to the control group, Superscript ‘b’: Red puncta compared to the control group. For (A, B) , 2-way ANOVA Dunnett’s multiple comparisons tests were used; for (D) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used. Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fenofibrate suppresses Mycoplasma bovis infection via autophagy-mediated cholesterol regulation in bovine mammary epithelial cells and murine mammary tissue

    doi: 10.3389/fcimb.2025.1731492

    Figure Lengend Snippet: Fenofibrate restores autophagic activity and autophagic flux in bMECs infected with M. bovis . (A) bMECs were divided into 6 experimental groups: control, M. bovis PG45 reference strain infection (MOI = 30, 9 hpi), M. bovis WT21 wild-type strain infection (MOI = 30, 9 hpi), fenofibrate treatment alone, fenofibrate combined with PG45 infection, and fenofibrate combined with WT21 infection. Expression levels of LAMP2, RAB7A, SQSTM1, and LC3, along with the loading control GAPDH, were evaluated by Western blot (using specific antibodies). (B) Densitometric analysis was performed to quantify relative protein levels of LAMP2, RAB7A, SQSTM1, and LC3-II, normalized to GAPDH. (C) Representative confocal images depict autophagic flux in mCherry-GFP-LC3-transfected bMECs across the 6 treatment groups: control, PG45-infected, WT21-infected, fenofibrate-treated, fenofibrate+PG45, and fenofibrate+WT21. Yellow puncta indicate autophagosomes, whereas red puncta represent autolysosomes. Nuclei were counterstained with Hoechst 33258 (blue). Scale bar = 10 μm. (D) Quantification of autophagosomes in bMECs. Twenty cells for each sample and at least 60 cells in each group were used for statistical analyses, Superscript ‘a’: Yellow puncta compared to the control group, Superscript ‘b’: Red puncta compared to the control group. For (A, B) , 2-way ANOVA Dunnett’s multiple comparisons tests were used; for (D) , 1-way ANOVA Dunnett’s multiple comparisons tests and 2-tailed unpaired t -tests were used. Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: Anti-LAMP1 antibody (67300-1-Ig), anti- lysosomal-associated membrane protein 2 (LAMP2) antibody (66301-1-Ig), anti-TFEB antibody (13372-1-AP), anti-TFE3 antibody (14480-1-AP), anti-LC3 polyclonal antibody (14600-1-AP), anti-ATG5 antibody (10181-2-AP), anti-RAB7A antibody (55469-1-AP), anti-mouse IgG-horseradish peroxidase (HRP) (SA00001-1), and Goat anti-rabbit IgG (SA00001-2) were all from Proteintech (Chicago, IL, USA).

    Techniques: Activity Assay, Infection, Control, Expressing, Western Blot, Transfection

    Fenofibrate affects expression of lysosome markers in mammary tissue of mice infected with M. bovis . (A) Mice were allocated into 6 groups: control, M. bovis PG45-infected, M. bovis WT21 wild-type strain-infected, fenofibrate-treated, fenofibrate + PG45-infected, and fenofibrate + WT21-infected. Immunohistochemical staining was used to detect LAMP1 expression in murine mammary tissue. LAMP1-positive granule-like cells were observed under a light microscope, with brown staining in nuclei indicating positive signals. (B) Quantification of LAMP1-positive staining intensity in mammary tissues. (C) Using the same 6 experimental groups, immunohistochemical staining was performed to assess LAMP2 expression in mammary tissue. LAMP2-positive granule-like cells were observed under a light microscope, with brown nuclear staining indicating positive expression. (D) Quantification of LAMP2-positive staining intensity in mammary tissues. Scale bar = 10 μm. For (B, D) , 2-way ANOVA Dunnett’s multiple comparisons tests were used. Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Fenofibrate suppresses Mycoplasma bovis infection via autophagy-mediated cholesterol regulation in bovine mammary epithelial cells and murine mammary tissue

    doi: 10.3389/fcimb.2025.1731492

    Figure Lengend Snippet: Fenofibrate affects expression of lysosome markers in mammary tissue of mice infected with M. bovis . (A) Mice were allocated into 6 groups: control, M. bovis PG45-infected, M. bovis WT21 wild-type strain-infected, fenofibrate-treated, fenofibrate + PG45-infected, and fenofibrate + WT21-infected. Immunohistochemical staining was used to detect LAMP1 expression in murine mammary tissue. LAMP1-positive granule-like cells were observed under a light microscope, with brown staining in nuclei indicating positive signals. (B) Quantification of LAMP1-positive staining intensity in mammary tissues. (C) Using the same 6 experimental groups, immunohistochemical staining was performed to assess LAMP2 expression in mammary tissue. LAMP2-positive granule-like cells were observed under a light microscope, with brown nuclear staining indicating positive expression. (D) Quantification of LAMP2-positive staining intensity in mammary tissues. Scale bar = 10 μm. For (B, D) , 2-way ANOVA Dunnett’s multiple comparisons tests were used. Data are presented as mean ± SD from 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: Anti-LAMP1 antibody (67300-1-Ig), anti- lysosomal-associated membrane protein 2 (LAMP2) antibody (66301-1-Ig), anti-TFEB antibody (13372-1-AP), anti-TFE3 antibody (14480-1-AP), anti-LC3 polyclonal antibody (14600-1-AP), anti-ATG5 antibody (10181-2-AP), anti-RAB7A antibody (55469-1-AP), anti-mouse IgG-horseradish peroxidase (HRP) (SA00001-1), and Goat anti-rabbit IgG (SA00001-2) were all from Proteintech (Chicago, IL, USA).

    Techniques: Expressing, Infection, Control, Immunohistochemical staining, Staining, Light Microscopy